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. 1996 Oct 1;93(20):10614-9.
doi: 10.1073/pnas.93.20.10614.

Parallel human genome analysis: microarray-based expression monitoring of 1000 genes

M Schena  1 , D Shalon, R Heller, A Chai, P O Brown, R W Davis
Affiliations

Parallel human genome analysis: microarray-based expression monitoring of 1000 genes

M Schena et al. Proc Natl Acad Sci U S A. .

Abstract

Microarrays containing 1046 human cDNAs of unknown sequence were printed on glass with high-speed robotics. These 1.0-cm2 DNA "chips" were used to quantitatively monitor differential expression of the cognate human genes using a highly sensitive two-color hybridization assay. Array elements that displayed differential expression patterns under given experimental conditions were characterized by sequencing. The identification of known and novel heat shock and phorbol ester-regulated genes in human T cells demonstrates the sensitivity of the assay. Parallel gene analysis with microarrays provides a rapid and efficient method for large-scale human gene discovery.

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