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. 1977 Jul;13(7):423-8.
doi: 10.1007/BF02615102.

Organ culture of human articular cartilage: studies on sulphated glycosaminoglycan synthesis

L S McKenzie, B A Horsburgh, P Ghosh, T K Taylor

Organ culture of human articular cartilage: studies on sulphated glycosaminoglycan synthesis

L S McKenzie et al. In Vitro. 1977 Jul.

Abstract

An organ culture system is described for adult human articular cartilage obtained from joints after femoral head replacement operations. Cartilage slices maintain maximal viability for 2 days in culture as assessed by uptake of [3H]uridine and [3H]leucine into whole tissue, and 35SO4 into sulphated glycosaminoglycans (GAGs). Since GAGs are the components of cartilage matrix, the depletion of which is associated with osteoarthrosis, a method for measuring sulphated GAG synthesis in culture has been investigated.

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