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. 1996 Sep;34(9):2205-9.
doi: 10.1128/jcm.34.9.2205-2209.1996.

Techniques for investigation of an apparent outbreak of infections with Candida glabrata

S Arif  1 , T Barkham, E G Power, S A Howell
Affiliations

Techniques for investigation of an apparent outbreak of infections with Candida glabrata

S Arif et al. J Clin Microbiol. 1996 Sep.

Abstract

A cluster of Candida glabrata isolates recovered from seven patients in an intensive care unit over a 10-week period were compared with a collection of isolates from six epidemiologically distinct outpatients and a reference strain by several DNA typing methods. Restriction enzyme analysis with HinII distinguished 13 strains from the 14 sources and was the method of choice. Pulsed-field gel electrophoresis and random amplification of polymorphic DNA both detected nine types from the 14 sources; however, the results of these two methods did not always correlate. These methods demonstrated that five of the seven patients had distinguishable strains and that cross-infection was unlikely.

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