doi: 10.1007/BF00368297.
Detection of methyltransferase activities which modify Gppp G to m7GpppGm in embryonic chick lens
- PMID: 887098
- DOI: 10.1007/BF00368297
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Detection of methyltransferase activities which modify Gppp G to m7GpppGm in embryonic chick lens
Mol Biol Rep.
1977 Jun.
Abstract
GpppG was modified to m7 GpppGm by a cytoplasmic extract, prepared from embryonic lens cells, in a reaction mixture which contained S-adenosyl-methionine as methyl group donor. The appearance of m7 GpppGm was a function of time and lens extract concentration. S-adenosyl-homocysteine inhibited both the m7G and Gm modification reactions. Analogues of GpppG, pG, ppG and pppG were relatively ineffective as substrates.
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