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. 1979 Jun 25;6(8):2849-61.
doi: 10.1093/nar/6.8.2849.

Detection of AKR MuLV-specific RNA in AKR mouse cells by in situ hybridization

C Godard, K W Jones
Free PMC article

Detection of AKR MuLV-specific RNA in AKR mouse cells by in situ hybridization

C Godard et al. Nucleic Acids Res. .
Free PMC article

Abstract

Conditions for the detection of complex RNA sequences by in situ hybridization have been investigated by using a single-stranded 3H-cDNA probe complementary to the AKR MuLV genome and in vitro cultured AKR mouse cells which spontaneously produce AKR MuLV. It is shown that fixation with glutaraldehyde at low concentration allows cellular RNA to be sufficiently well retained during the annealing process and that stringent conditions in situ can be maintained by means of formamide. Some conditions which promote atypical and non-specific binding of the probe have been identified.

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References

    1. Nucleic Acids Res. 1974 Jun;1(6):787-801 - PubMed
    1. Virology. 1975 Jan;63(1):40-7 - PubMed
    1. Cold Spring Harb Symp Quant Biol. 1975;39 Pt 2:1085-101 - PubMed
    1. Int J Cancer. 1975 Dec 15;16(6):998-1007 - PubMed
    1. Nucleic Acids Res. 1977;4(5):1539-52 - PubMed