Fluorogenic and chromogenic enzyme substrates in culture media and identification tests

Abstract

Rapid detection and identification of microorganisms is extremely important in many fields of applied and research microbiology. In general, fluorogenic and chromogenic substrates have proved to be a powerful tool, utilizing specific enzymatic activities of certain microorganisms, either in parallel with or instead of traditional methods. By incorporation of synthetic fluorogenic or chromogenic substrates into primary selective media, enumeration and detection can be performed directly on the isolation plate. The introduction of many of these media and identification tests has led to improved accuracy and faster detection of target organisms, often reducing the need for isolation of pure cultures and confirmatory tests.