Cell biology of kidney glomerulus

Abstract

It has been accepted that some artifacts are inevitably produced by the conventional preparation steps for electron microscopy, including fixation, dehydration, embedding, ultrathin sectioning, and staining. Therefore, conventional ultrastructural findings on kidney glomeruli are hardly thought to be correlated with the physiological functions of kidneys in vivo. In this chapter, two preparation techniques, the quick-freezing and deep-etching (QF-DE) method or the quick-freezing and freeze-substitution (QF-FS) method, are presented and shown to be useful for clarifying the ultrastructures of kidney glomeruli more closely to structures in vivo with fewer artifacts. Moreover, the ultrastructures of glomerular capillary loops have been demonstrated by a new "in vivo cryotechnique," that shows that hemodynamic factors should be considered in the morphological study of glomerular functions.