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. 1996 Jan;11(1):74-81.
doi: 10.3904/kjim.1996.11.1.74.

PGF2 alpha causes bronchoconstriction and pulmonary vasoconstriction via thromboxane receptors in rat lung

K H Kang  1 , J J Shim, M Banerjee, J H Newman
Affiliations

PGF2 alpha causes bronchoconstriction and pulmonary vasoconstriction via thromboxane receptors in rat lung

K H Kang et al. Korean J Intern Med. 1996 Jan.

Abstract

We determined the vascular and airway effects of PGF2 alpha and its mechanism of action on isolated-perfused lungs of rats were isolated and perfused at 50 ml/kg/min with Krebs-Henseleit bicarbonate buffer solution containing 3% bovine serum albumin. The lungs were ventilated with 21% O2 and 5% CO2 at a tidal volume of 2 ml. frequency of 60 per minute and positive end expiratory pressure of 3 cmH2O. Following injection of 50 micrograms PGF2 alpha into the afferent pulmonary catheter, there was a marked rise in pulmonary arterial pressure (Ppa) and in resistance to airflow across the lung (RL) and a fall in dynamic lung compliance (Cdyn). Double vascular occlusion technique revealed that 29% of the rise in Ppa was due to an increase in upstream and 71% to downstream resistance. N omega-nitro-L-arginine, 100 microns, a NO synthase inhibitor potentiated the Ppa response two-fold with significant change in airway mechanics. Rat atrial natriuretic factor (r-ANF), 40 micrograms quickly reversed the changes in Ppa, RL and Cdyn. Infusion of r-ANF prior to PGF2 alpha attenuated the Ppa response by 38%, RL by 44% and Cdyn by 12%. SQ 29548, a thromboxane receptor blocker and Cl, a protein kinase C (PKC) inhibitor, fully blocked both the vascular and airway responses to PGF2 alpha. PGF2 alpha is a constrictor of pulmonary vessels and airways in rat lungs via thromboxane SQ 29548 receptors, thansduced by intracellular PKC.

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Figures

Fig. 1.
Fig. 1.
Change in longitudinal vascular segment pressure to a bolus dose of 50 μg PGF2α. in 15 isolated-perfused rat lungs, measured by double occlusion. PPA, pulmonary arterial pressure: PmV, microvascular pressure: PPA-PmV, difference. Most of the pressure change was in the downstream segment.
Fig. 2.
Fig. 2.
Pulmonary arterial pressure response to PGF2α without and with pre-treatment with atrial natriuretic factor(ANF). Values are means±SE of 6 experiments.
Fig. 3.
Fig. 3.
Pulmonary arterial pressure response to PGF2α without and with pretreatment with Nw-nitro-L-arginine(NNLA). Values are means±SE of 12 experiments.
See this image and copyright information in PMC

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