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. 1996 Oct 3;174(2):221-4.
doi: 10.1016/0378-1119(96)00083-2.

Isolation and characterization of a bovine gene encoding phenol sulfotransferase

T Henry  1 , B Kliewer, R Palmatier, J S Ulphani, J D Beckmann
Affiliations

Isolation and characterization of a bovine gene encoding phenol sulfotransferase

T Henry et al. Gene. .

Abstract

Phenol sulfotransferases (PST) esterify hydroxylated aromatic compounds with sulfate, and therefore play a role in the metabolism of xenobiotics. In this investigation, a bovine PST gene of 2372 bp was amplified from genomic DNA. Sequence overlap with the cognate cDNA revealed seven exons, with all introns containing GT/AG splicing donor/acceptor site. This product was subcloned into an SV40-based expression vector and transfected into WI-26 human lung fibroblasts. Immunoblot analyses revealed production of the anticipated 32-kDa protein, and the active enzyme displayed steady-state kinetic properties consistent with the enzyme characterized in bovine lung (apparent Km = 6.5 microM for 2-naphthol).

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