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. 1996 Nov;178(21):6357-60.
doi: 10.1128/jb.178.21.6357-6360.1996.

Apramycin resistance as a selective marker for gene transfer in mycobacteria

E Paget  1 , J Davies
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Apramycin resistance as a selective marker for gene transfer in mycobacteria

E Paget et al. J Bacteriol. 1996 Nov.

Abstract

We have explored the potential of using the apramycin resistance gene as a marker in mycobacterial gene transfer studies. Shuttle plasmids available for both electroporation and conjugation studies have been constructed, and we have successfully validated the use of the apramycin resistance gene as a component of cloning vectors for Mycobacterium smegmatis, M. bovis BCG, and M. tuberculosis.

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