RNAs of influenza virus recombinants derived from parents of known virulence for man

Abstract

Extensive use of recombinants made from A/PR/8/34 (H0N1) and wild, virulent H3N2 viruses as live influenza vaccines has provided a number of viruses of defined virulence for man. Clinical symptoms produced by these strains have ranged from febrile influenza to local coryzal symptoms or nil. A study was therefore made of the extent to which the PR8 genome had been incorporated into that of a number of the recombinants. By RNA--RNA hybridization it seemed that recombinants which had 55 per cent of greater homology with the PR8 parent were likely to conform an acceptable standard of attenuation. Those with lesser homology were frequently, but not always, clinically virulent. The technique seemed potentially useful, therefore, for screening PR8 live vaccine recombinants in vitro before giving them to volunteers.