Ruminant cluster CD14

Abstract

Following the analysis of flow cytometry data on ovine and bovine cells and immunoprecipitation, immunohistochemistry and competitive binding studies, a cluster of three monoclonal antibodies (mAbs) (VPM65, VPM66 and VPM67) appeared to be specific for the ruminant CD14 (Gupta, V.K., McConnell, I., Dalziel, R.G. and Hopkins, J., 1996. Identification of the sheep homologue of the monocyte cell surface molecule-CD14. Vet. Immunol. Immunopathol. in press; Hopkins, J. and Gupta, V.K., 1996. Characterization of 3rd Worshop monoclonal antibodies specific for sheep macrophages/monocytes. Vet. Immunol. Immunopathol., 52:329-339). According to flow cytometry results from human CD14-transfected COS-7 cells and from bovine peripheral blood mononuclear cells, another mAb (CC-G33), not submitted to the Workshop, recognised a bovine CD14 epitope which is conserved in the human CD14 (Sopp, P., Kwong, L.S. and Howard, C.J., 1996. Identification of bovine CD14. Vet. Immunol. Immunopathol., 52: 323-328).