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. 1996 Nov;34(11):2843-6.
doi: 10.1128/jcm.34.11.2843-2846.1996.

Specificity of IS6110-based amplification assays for Mycobacterium tuberculosis complex

T J Hellyer  1 , L E DesJardin, M K Assaf, J H Bates, M D Cave, K D Eisenach
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Specificity of IS6110-based amplification assays for Mycobacterium tuberculosis complex

T J Hellyer et al. J Clin Microbiol. 1996 Nov.

Abstract

The specificity of IS6110 for the Mycobacterium tuberculosis complex has recently been questioned. We observed no cross-reaction with 27 nontuberculous mycobacteria using strand displacement- and PCR-based amplification of the nucleotide 970 to 1026 and 762 to 865 regions of IS6110. These data support use of selected regions of IS6110 as M. tuberculosis complex-specific targets.

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