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. 1996 Nov;62(11):4233-7.
doi: 10.1128/aem.62.11.4233-4237.1996.

Neomycin resistance as a selectable marker in Methanococcus maripaludis

J L Argyle  1 , D L Tumbula, J A Leigh
Affiliations

Neomycin resistance as a selectable marker in Methanococcus maripaludis

J L Argyle et al. Appl Environ Microbiol. 1996 Nov.

Abstract

We cloned the aminoglycoside phosphotransferase genes APH3'I and APH3'II between the Methanococcus voltae methyl reductase promoter and terminator in a plasmid containing a fragment of Methanococcus maripaludis chromosomal DNA. The resulting plasmids encoding neomycin resistance transformed M. maripaludis at frequencies similar to those observed for pKAS102 encoding puromycin resistance. The antibiotic geneticin was not inhibitory to M. maripaludis.

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References

    1. Mol Gen Genet. 1990 Apr;221(2):273-9 - PubMed
    1. Sex Transm Dis. 1986 Jul-Sep;13(3 Suppl):185-8 - PubMed
    1. Annu Rev Microbiol. 1992;46:165-91 - PubMed
    1. Appl Environ Microbiol. 1983 Jul;46(1):220-6 - PubMed
    1. Appl Environ Microbiol. 1994 Mar;60(3):903-7 - PubMed

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