Neurogenesis in the developing crab brain: postembryonic generation of neurons persists beyond metamorphosis

Abstract

A considerable amount of information is available about the structure and function of the central nervous system in adult crustaceans. However, little effort has been directed toward understanding embryonic and larval neurogenesis in these animals. In the present study we recorded neurogenesis in the brain of laboratory-reared larvae of the spider crab Hyas araneus. Proliferating cells were detected immunocytochemically after in vivo labeling with 5-bromo-2'-deoxyuridine. This method has already been used to study the proliferation of neuroblasts in the ventral nerve cord of spider crab larvae. In the brain, a set of mitotically highly active neuroblasts was found in newly hatched zoea 1 larvae. These neuroblasts are individually identifiable due to their position and therefore a schematic map of the cerebral neuroblasts could be established. The number of active neuroblasts is high from hatching throughout the molt to the zoea 2. This proliferative action then decreases dramatically and has ceased at the time of first metamorphosis toward the megalopa larva. However, many ganglion mother cells born by unequal division of neuroblasts then go through their final division throughout the subsequent megalopa stage. In the brain, all mitotic activity has ceased at the time of second metamorphosis with the exception of a cluster of labeled nuclei within the olfactory lobe cells. In this cluster, the generation of neurons persists beyond the second metamorphosis into the crab 1 stage. Meanwhile, the neuropil volume of the olfactory lobes increases 10-fold from hatching to the crab 1. These results are discussed with regard to reports on neuronal proliferation during adult life in insects and rodents.