Change of karyoskeleton during mammalian spermatogenesis: expression pattern of nuclear lamin C2 and its regulation

Abstract

Nuclear lamins are a multigene family of major karyoskeletal proteins. The expression pattern of members of the lamin family has been shown to be developmentally regulated. Of particular interest have been the findings that mammalian spermatogenic cells express two small lamin isoforms (B3 and C2) which are shorter splicing variants of their somatic counterparts (the lamins B2 and C, respectively). Although lamins B3 and C2 appear to be specific for the germ line, the expression pattern and localization of these proteins is not known. In the present study, we present evidence that during rat spermatogenesis lamin C2 is selectively expressed in spermatocytes, i.e., the cells undergoing meiosis. As in the case of other members of the family, lamin C2 was detected at the nuclear periphery. Northern blotting and in situ hybridization indicate that meiotic expression of lamin C2 is regulated at the transcriptional level. This is in contrast to the situation during amphibian oogenesis, where lamin expression is largely posttrascriptionally regulated. Interestingly, the expression pattern of lamin C2 temporally coincides with that of structural protein components of another meiosis-specific karyoskeletal structure, the synaptonemal complex (SC). Taken together, we conclude that pairing and recombination of homologous chromosomes during meiotic prophase is accompanied by significant changes in the organization of the karyoskeleton which are accomplished by the expression of stage-specific proteins.