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Comparative Study
. 1996 Sep;15(9):749-52.
doi: 10.1007/BF01691965.

Comparison of immunofluorescence with enzyme immunoassay for detection of Q fever

S C D'Harcourt  1 , A B Soto, V C Burgos, D L Calero, R Martínez-Zapico
Affiliations
Comparative Study

Comparison of immunofluorescence with enzyme immunoassay for detection of Q fever

S C D'Harcourt et al. Eur J Clin Microbiol Infect Dis. 1996 Sep.

Abstract

To evaluate enzyme immunoassay (EIA) as an alternative to indirect immunofluorescence assay (IFA) to screen for Q fever in humans, 157 serum samples from patients suspected of having the disease were tested for immunoglobulin G antibodies to Coxiella burnetii. The agreement between the tests and the sensitivity of EIA were excellent (96.8% and 98.4%, respectively) when an IFA titer of > 1/160 was considered positive. All serum samples with a titer of > 1/320 in the IFA were also positive by the EIA. The EIA seems to be an acceptable alternative to IFA for screening for Q fever.

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References

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