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. 1995 Nov 3;673(1):15-26.
doi: 10.1016/0378-4347(95)00241-a.

Separation of major polar lipids in Pecten maximus by high-performance liquid chromatography and subsequent determination of their fatty acids using gas chromatography

P Soudant  1 , Y Marty, J Moal, J F Samain
Affiliations

Separation of major polar lipids in Pecten maximus by high-performance liquid chromatography and subsequent determination of their fatty acids using gas chromatography

P Soudant et al. J Chromatogr B Biomed Appl. .

Abstract

An easy method for the separation of major polar lipid classes by HPLC is described. Maximum resolution was achieved by an automated combination of a silica gel column and a diol column. Polar lipid analysis of the larvae and gonads of Pecten maximus showed the presence of a particular glycolipid especially rich in 22:6(n - 3) and the predominance of 20:4(n - 6) in the phosphatidylinositol. The phosphatidylcholine and phosphatidylethanolamine (diacyl form + alkenylacyl) were the major fractions. The plasmalogen form (25% in larvae, 34% in gonads) was essentially composed of polyunsaturated fatty acids of 20 and 22 carbons in the sn-2 position.

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