[Detection of the spring viremia of carp virus by hybridization with nonradioactive DNA probes]

Abstract

In order to detect spring viraemia of carp virus, DNA probes have been constructed using reverse transcription-PCR amplification technique and cDNA cloning in plasmid and phage vectors. The sensitivity and specificity of viral RNA detection was assessed using nonradioactive probes in infected FHM cell culture and in tissues from dead fish. Viral RNA was more frequently detected in the brain and gill than in the abdominal organs.