Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1996 Mar 15;137(1):75-8.
doi: 10.1111/j.1574-6968.1996.tb08085.x.

Purification and characteristics of cytosolic chitinase from Piromyces communis OTS1

M Sakurada  1 , D P Morgavi, K Komatani, Y Tomita, R Onodera
Affiliations

Purification and characteristics of cytosolic chitinase from Piromyces communis OTS1

M Sakurada et al. FEMS Microbiol Lett. .

Abstract

A chitinase was purified from the cytosolic fraction of the anaerobic rumen fungus Piromyces communis OTS1 by affinity chromatography using regenerated chitin, gel filtration and chromatofocusing. The chitinase was most active at pH 6.2 and at 60 degrees C in a 20-min assay. The molecular mass of the purified protein was estimated by SDS-PAGE to be 42 kDa and its pI was 4.9. The enzyme activity, which was of the 'endo' type, was inhibited by Ag+, Hg2+ and allosamidin. N-Acetyl-beta-glucosaminidase and 'exo' type chitinase activity were absent from the purified preparation.

PubMed Disclaimer

Publication types