Dansyl hydrazine as a fluorimetric reagent for thin-layer chromatographic analysis of reducing sugars
- PMID: 893622
- DOI: 10.1016/s0021-9673(00)89330-9
Dansyl hydrazine as a fluorimetric reagent for thin-layer chromatographic analysis of reducing sugars
Abstract
Reducing sugars, particularly aldoses, react readily with dansyl hydrazine. The fluorescent hydrazones produced can be separated by thin-layer chromatography and determined quantitatively by spectrofluorimetry after elution from the chromatograms.
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