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Case Reports
. 1979 Aug 18;2(8138):323-5.
doi: 10.1016/s0140-6736(79)90342-8.

Identification of a clinical isolate as Legionella pneumophila by gas chromatography and mass spectrometry of cellular fatty acids

S Fisher-Hoch, M J Hudson, M H Thompson
Case Reports

Identification of a clinical isolate as Legionella pneumophila by gas chromatography and mass spectrometry of cellular fatty acids

S Fisher-Hoch et al. Lancet. .

Abstract

Culture of a bronchial aspirate from an immunosuppressed patient with severe pneumonia yielded a growth of filamentous poorly staining gram-negative rods. Fluorescence with Legionella pneumophila direct fluorescein-isothiocyanate conjugate was equivocal. Gas-liquid chromatography (GLC) and GLC/mass-spectrometry (GLC-MS) of the cellular fatty acids of the isolate confirmed that the organism was L. pneumophila. GLC and GLC-MS constitute a rapid and definitive method for identification of L. pneumophila isolates.

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