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. 1996 Nov 15;320 ( Pt 1)(Pt 1):187-92.
doi: 10.1042/bj3200187.

Kinetics of inactivation of bovine pancreatic ribonuclease A by bromopyruvic acid

M H Wang  1 , Z X Wang, K Y Zhao
Affiliations

Kinetics of inactivation of bovine pancreatic ribonuclease A by bromopyruvic acid

M H Wang et al. Biochem J. .

Abstract

The kinetic theory of substrate reaction during the modification of enzyme activity [Duggleby (1986) J. Theor. Biol. 123, 67-80; Wang and Tsou (1990) J. Theor. Biol. 142, 531-549] has been applied to a study of the inactivation kinetics of ribonuclease A by bromopyruvic acid. The results show that irreversible inhibition belongs to a non-competitive complexing type inhibition. On the basis of the kinetic equation of substrate reaction in the presence of the inhibitor, all microscopic kinetic constants for the free enzyme, the enzyme-substrate complex and the enzyme-product complex have been determined. The non-competitive inhibition type indicates that neither the substrate nor the product affects the binding of bromopyruvic acid to the enzyme and that the ionization state of His-119 may be the same in both the enzyme-substrate and the enzyme-product complexes.

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References

    1. Eur J Biochem. 1987 Jan 15;162(2):271-4 - PubMed
    1. J Theor Biol. 1990 Aug 23;145(4):547-72 - PubMed
    1. J Theor Biol. 1986 Nov 7;123(1):67-80 - PubMed
    1. Biochim Biophys Acta. 1987 Dec 18;916(3):465-73 - PubMed
    1. J Theor Biol. 1987 Aug 7;127(3):253-70 - PubMed

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