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. 1996 Sep-Oct;81(5):404-9.

Human umbilical cord blood: immunophenotypic heterogeneity of CD34+ hematopoietic progenitor cells

G D'Arena  1 , P Musto, N Cascavilla, G Di Giorgio, F Zendoli, M Carotenuto
Affiliations
  • PMID: 8952152

Human umbilical cord blood: immunophenotypic heterogeneity of CD34+ hematopoietic progenitor cells

G D'Arena et al. Haematologica. 1996 Sep-Oct.

Abstract

Background: Human umbilical cord blood (HUCB) is a possible alternative to bone marrow (BM) and mobilized peripheral blood (PB) for transplantation of hematopoietic progenitors. The aim of this study was to evaluate the phenotypic profile of CD34+ progenitors present in HUCB.

Materials and methods: A flow cytometric analysis was performed on 20 HUCB samples, using a large panel of monoclonal antibodies recognizing different lineage or activation antigens, in double labeling with CD34.

Results: A toal of 13,897 +/- 2,529 cells/microL, 0.84 +/- 0.83% of which were CD34+, was found. The large majority of CD34+ cells were committed toward initial myeloid differentiation (CD33+, CD13+) and expressed the transferrin receptor (CD71). A substantial proportion of these cells (about 40%) co-expressed CD45RA and CD117, while a very small number displayed markers of advanced myeloid commitment, such as CD14, CD15 and CD41 (less than 2%), or those of lymphoid differentiation: CD2, CD5, CD7, CD10 and CD19 (less than 6%). About 11% of HUCB CD34+ cells were primitive progenitors, as suggested by the absence of HLA-DR and CD38 on their surface.

Conclusions: As previously observed in BM and mobilized PB, the phenotype of HUCB CD34+ cells is quite heterogeneous. In particular, HUCB contains subpopulations of both early and committed hematopoietic progenitors which may represent a valid source for transplantation.

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