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. 1996 Sep;14(13):1205-13.
doi: 10.1016/s0264-410x(96)00032-1.

Physicochemical and immunological studies on the stability of free and microsphere-encapsulated tetanus toxoid in vitro

D K Xing  1 , D T Crane, B Bolgiano, M J Corbel, C Jones, D Sesardic
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Physicochemical and immunological studies on the stability of free and microsphere-encapsulated tetanus toxoid in vitro

D K Xing et al. Vaccine. 1996 Sep.

Abstract

The stability of tetanus toxoid released from experimental, slow-release microsphere vaccines was compared with that of free toxoid under different conditions over a 3 month period. The amount of antigenicity remaining, as measured by ELISA, correlated well with loss of toxoid structure, as determined by circular dichroism and fluorescence spectroscopy. At 37 degrees C and low pH, pH 2.5 for free toxoid, or under the acidic conditions generated by the hydrolysis of fast-release microspheres, a gradual unfolding of the polypeptide chain was observed within the first few weeks with more rapid denaturation beyond 30 days.

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