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. 1977 Jun;4(6):1911-31.
doi: 10.1093/nar/4.6.1911.

Conformational states of chromatin nu bodies induced by urea

D E Olins, P N Bryan, R E Harrington, W E Hill, A L Olins
Free PMC article

Conformational states of chromatin nu bodies induced by urea

D E Olins et al. Nucleic Acids Res. 1977 Jun.
Free PMC article

Abstract

Monomer chromatin nu bodies (nu1) from chicken erythrocyte nuclei were exposed to 0-10 M urea plus 0.2 mM EDTA (PH 7). Alterations in nu1 conformation were examined using hydrodynamic methods (i.e., S, eta, and (formula: see text)), thermal denaturation, circular dichroism, reactivity of histone thiol groups to N-ethyl maleimide, and electron microscopy. The two domains of a nu body (i.e., the DNA-rich shell and the protein-rich core) aeared to respond differently to the destabilizing effects of increasing urea; DNA conformation and stability exhibited noncooperative changes; the core protein structure revealed cooperative destabilization between 4 and 7 M urea. Companion studies on the conformation of the inner histone "heterotypic tetramer" also revealed cooperative destabilization with increasing urea concentration.

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References

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