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. 1996 Jun;22(6):425-31.

[Cloning cDNA for the ha-SDGF gene from a Syrian hamster cell line with increased metastatic potential using subtractive hybridization]

[Article in Russian]
N G Gurskaia, D A Shagin, K A Luk'ianov, L L Vagner, M S Shtutman, E A Musatkina, E V Moinova, A G Tatosian, S A Luk'ianov, E D Sverdlov
  • PMID: 8975671

[Cloning cDNA for the ha-SDGF gene from a Syrian hamster cell line with increased metastatic potential using subtractive hybridization]

[Article in Russian]
N G Gurskaia et al. Bioorg Khim. 1996 Jun.

Abstract

Using subtractive hybridization, a cDNA library containing over 50% of clones specific for a highly metastatic cell line was obtained from two hamster embryo fibroblast lines with different metastatic potentials. Most of the clones (83%) contained new sequences. One clone contained the ha-SDGF gene cDNA homologous to SDGF cDNA from rodents. The level of ha-SDGF mRNA expression was considerably higher in the highly metastatic cell line.

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