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. 1997 Jan;63(1):310-3.
doi: 10.1128/aem.63.1.310-313.1997.

A nucleic acid sequence-based amplification system for detection of Listeria monocytogenes hlyA sequences

B W Blais  1 , G Turner, R Sooknanan, L T Malek
Affiliations

A nucleic acid sequence-based amplification system for detection of Listeria monocytogenes hlyA sequences

B W Blais et al. Appl Environ Microbiol. 1997 Jan.

Abstract

A nucleic acid sequence-based amplification system primarily targeting mRNA from the Listeria monocytogenes hlyA gene was developed. This system enabled the detection of low numbers (< 10 CFU/g) of L. monocytogenes cells inoculated into a variety of dairy and egg products after 48 h of enrichment in modified listeria enrichment broth.

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