doi: 10.1016/s0014-5793(96)01298-7.
The algorithm of estimation of the Km values for primers in DNA synthesis catalyzed by human DNA polymerase alpha
Affiliations
- PMID: 8980132
- DOI: 10.1016/s0014-5793(96)01298-7
Item in Clipboard
The algorithm of estimation of the Km values for primers in DNA synthesis catalyzed by human DNA polymerase alpha
FEBS Lett.
.
Free article
Abstract
DNA synthesis with various deoxyribo homo- and heterooligoprimers in the presence of complementary templates was investigated. The lengthening of d(pN)n, primers (n = 1-10) by a unit resulted in an increase of the primer affinity and a maximal rate of polymerization. The coefficient of the affinity enhancement of primers due to formation of one hydrogen bond between primer and template was found to be 1.35. The dependence of the primer affinity and polymerization rate on template-primer structure in solution was analyzed and the objective laws of the changes of the KM and Vmax values were revealed.
Similar articles
-
The algorithm of estimation of the Km values for primers of various structure and length in the polymerization reaction catalyzed by Klenow fragment of DNA polymerase I from E. coli.FEBS Lett. 1989 Nov 20;258(1):166-70. doi: 10.1016/0014-5793(89)81642-4. FEBS Lett. 1989. PMID: 2480250
-
Functional analysis of primers and templates in the synthesis of DNA catalyzed by human immunodeficiency virus type 1 reverse transcriptase.Eur J Biochem. 1992 Jul 1;207(1):351-8. doi: 10.1111/j.1432-1033.1992.tb17057.x. Eur J Biochem. 1992. PMID: 1378404
-
[Prokaryotic and eukaryotic DNA-polymerase. I. The role of internucleotide phosphate groups in the binding of a primer with the enzyme].Bioorg Khim. 1987 Jan;13(1):45-57. Bioorg Khim. 1987. PMID: 3551963 Russian.
-
Interactions of calf thymus DNA polymerase alpha with primer/templates.Nucleic Acids Res. 1995 Oct 25;23(20):4109-15. doi: 10.1093/nar/23.20.4109. Nucleic Acids Res. 1995. PMID: 7479073 Free PMC article.
-
[Comparison of the effectiveness of the interaction of ribo- and deoxyriboprimers with DNA-polymerase from the human placenta].Mol Biol (Mosk). 1987 Sep-Oct;21(5):1378-85. Mol Biol (Mosk). 1987. PMID: 3683380 Russian.
Cited by
-
Thermodynamic and kinetic basis for recognition and repair of 8-oxoguanine in DNA by human 8-oxoguanine-DNA glycosylase.Nucleic Acids Res. 2011 Jun;39(11):4836-50. doi: 10.1093/nar/gkq1333. Epub 2011 Feb 22. Nucleic Acids Res. 2011. PMID: 21343179 Free PMC article.
-
Uracil-DNA glycosylase: Structural, thermodynamic and kinetic aspects of lesion search and recognition.Mutat Res. 2010 Mar 1;685(1-2):11-20. doi: 10.1016/j.mrfmmm.2009.10.017. Epub 2009 Nov 10. Mutat Res. 2010. PMID: 19909758 Free PMC article. Review.
-
Thermodynamic, kinetic and structural basis for recognition and repair of abasic sites in DNA by apurinic/apyrimidinic endonuclease from human placenta.Nucleic Acids Res. 2004 Sep 30;32(17):5134-46. doi: 10.1093/nar/gkh846. Print 2004. Nucleic Acids Res. 2004. PMID: 15459284 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
