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. 1996 Mar;3(2):216-8.
doi: 10.1128/cdli.3.2.216-218.1996.

Detection of rubella virus-specific immunoglobulin M antibodies with a baculovirus-expressed E1 protein

M Schmidt  1 , C Lindqvist, A Salmi, C Oker-Blom
Affiliations

Detection of rubella virus-specific immunoglobulin M antibodies with a baculovirus-expressed E1 protein

M Schmidt et al. Clin Diagn Lab Immunol. 1996 Mar.

Abstract

The structural proteins of rubella virus (RV) were expressed in insect cells by using the baculovirus expression vector system. The recombinant E1 envelope glycoprotein was purified by immunoaffinity chromatography and used to detect RV-specific immunoglobulin M antibodies in a time-resolved fluoroimmunoassay. Correlation analysis between the reactivities of antibodies against this recombinant E1 and the reactivities against authentic RV antigen shows that purified E1 can detect RV antibodies of the immunoglobulin M type.

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