Localization of nitric oxide synthase in the brain of the frog, Xenopus laevis

Abstract

Nitric oxide synthase was localized in the brain of the South african clawed frog by NADPH diaphorase histochemistry and immunohistochemistry. All structures stained by the antiserum also displayed NADPH diaphorase activity. The fiber bundles of the terminal nerve, however, were positive for NADPH diaphorase but were not immunoreactive. In the forebrain, neurons expressing nitric oxide synthase were concentrated to the pallium, striatum, nucleus accumbens and anterior entopeduncular nucleus. Strongly stained neurons in the diencephalon were detected in the lateral thalamus, the tuberculum posterior and in the ventral hypothalamus. In the mesencephalon, the tectum and the magnocellular nucleus of the torus semicircularis contained many positive cells. Farther caudally, intensely stained neurons were abundant in an area corresponding to the anuran locus coeruleus, in the descending nucleus of the trigeminus and the inferior reticular nucleus. In the cerebellum, Purkinje cells were weakly stained. In summary, the expression pattern of nitric oxide synthase in the anuran brain reveals similarities to that of other vertebrates. The strongly positive cell group in the locus coeruleus may correspond to cholinergic cell groups in the mesopontine area in mammals.