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Comparative Study
. 1997 Feb;35(2):525-6.
doi: 10.1128/jcm.35.2.525-526.1997.

Quantitation of cytomegalovirus (CMV) DNA in leukocytes of human immunodeficiency virus-infected subjects with and without CMV disease by using PCR and the SHARP Signal Detection System

G Boivin  1 , J Handfield, G Murray, E Toma, R Lalonde, J G Lazar, M G Bergeron
Affiliations
Comparative Study

Quantitation of cytomegalovirus (CMV) DNA in leukocytes of human immunodeficiency virus-infected subjects with and without CMV disease by using PCR and the SHARP Signal Detection System

G Boivin et al. J Clin Microbiol. 1997 Feb.

Abstract

We report the development of a simple and rapid PCR assay for quantitation of the cytomegalovirus (CMV) DNA load in polymorphonuclear leukocytes. Using this system, a very good correlation was found between a high number of CMV copies in the blood and the presence of CMV disease in subjects with AIDS.

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