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. 1997 Feb;179(3):968-71.
doi: 10.1128/jb.179.3.968-971.1997.

Purification and in vitro phosphorylation of HupT, a regulatory protein controlling hydrogenase gene expression in Rhodobacter capsulatus

S Elsen  1 , A Colbeau, P M Vignais
Affiliations

Purification and in vitro phosphorylation of HupT, a regulatory protein controlling hydrogenase gene expression in Rhodobacter capsulatus

S Elsen et al. J Bacteriol. 1997 Feb.

Abstract

The HupT protein of Rhodobacter capsulatus, involved in negative regulation of hydrogenase gene expression, is predicted to be a histidine kinase on the basis of sequence comparisons. The protein was overproduced in Escherichia coli, purified to homogeneity, and demonstrated to autophosphorylate in vitro in the presence of [gamma-32P]ATP. An H217N hupt mutant was constructed, and the mutant protein was shown to have lost kinase activity. This result, and the fact that the phosphoryl group in phosphorylated HupT appeared to be bound to an N atom, support the suggestion from sequence comparisons that HupT is a histidine kinase, which can autophosphorylate on the His217 residue.

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References

    1. Anal Biochem. 1976 May 7;72:248-54 - PubMed
    1. J Bacteriol. 1997 Jan;179(1):290-2 - PubMed
    1. Cell. 1988 Apr 8;53(1):79-87 - PubMed
    1. Nucleic Acids Res. 1988 Aug 11;16(15):7351-67 - PubMed
    1. Proc Natl Acad Sci U S A. 1988 Dec;85(23):8919-23 - PubMed

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