Stabilized expression of mRNA is associated with mycobacterial resistance controlled by Nramp1

Abstract

Control of innate resistance to the growth of mycobacteria is mediated by a gene termed Nramp1. Although the role of the protein product of Nramp1 in mediating resistance to mycobacterial growth is not known, the effect of the gene is pleiotropic and it has been suggested that the gene controls macrophage priming for activation. We have found that the functional capacity of macrophages from Mycobacterium bovis BCG-susceptible mice can be suppressed by corticosterone, while the function of macrophages from BCG-resistant mice remains unaffected. In this study, we show that corticosterone differentially affects the stability of mRNAs of several recombinant gamma interferon (rIFN-gamma)-induced genes. Treatment of macrophages from BCG-susceptible mice with corticosterone accelerates the decay of Nramp1 mRNA. The mRNA of IFN-gamma-induced genes of macrophages from BCG-resistant mice was more stable than the mRNA of macrophages from BCG-susceptible mice in the presence or absence of corticosterone. The results of this investigation suggest that Nramp1 acts by stabilizing the mRNA of genes associated with macrophage activation, thus accounting for the functional differences that have been attributed to these macrophage populations.