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. 1996 Winter;6(4):267-72.
doi: 10.1089/oli.1.1996.6.267.

Resistance of morpholino phosphorodiamidate oligomers to enzymatic degradation

R M Hudziak  1 , E Barofsky, D F Barofsky, D L Weller, S B Huang, D D Weller
Affiliations

Resistance of morpholino phosphorodiamidate oligomers to enzymatic degradation

R M Hudziak et al. Antisense Nucleic Acid Drug Dev. 1996 Winter.

Abstract

Oligomers possessing the Morpholino phosphorodiamidate backbone were evaluated for resistance to a variety of enzymes and biologic fluids. A 25-mer was incubated with nucleases, proteases, esterases, and serum, and the reaction mixtures were directly analyzed by MALDI-TOF mass spectrometry. The 25-mer was completely resistant to 13 different hydrolases and serum and plasma. The excellent resistance of Morpholino phosphorodiamidates to enzymatic attack indicates their suitability for in vivo use.

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