Review
doi: 10.1016/s0958-1669(97)80165-6.
High-speed DNA sequencing in ultrathin slab gels
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- PMID: 9013648
- DOI: 10.1016/s0958-1669(97)80165-6
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Review
High-speed DNA sequencing in ultrathin slab gels
Curr Opin Biotechnol.
1997 Feb.
Abstract
There have been many recent theoretical and technical advances in the sequencing of DNA in ultrathin slab gels. These include recent experimental progress in four areas: DNA polymerases; DNA template preparation; the separation and detection of DNA bands; and base-calling algorithms. The Zimm-Lumpkin theory of electrophoresis provides a new and improved framework for understanding the operation of sequencing gels.
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