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. 1977 Oct 20;23(3):225-8.
doi: 10.1007/BF02012789.

A micromethod for the determination of fluoride in blood plasma and saliva

J Ekstrand

A micromethod for the determination of fluoride in blood plasma and saliva

J Ekstrand. Calcif Tissue Res. .

Abstract

A simple and accurate technique for the determination of fluoride (F-) in capillary-sampled blood is presented. The method is based on the known addition-slope determination technique using the fluoride electrode. No calibration curve or calibration of the electrode is required. A standard deviation of 1.3-5.6% in the range 300-10 ng F/ml was given by 259 duplicate determinations on human plasma. Measurements of parotid saliva showed that it was possible to analayze fluoride concentrations as low as 4 ng F/ml with good reproducibility. The results also show that a 150 microliter sample is sufficient for fluoride determination with high accurary. This method can easily be used in clinical laboratory.

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