Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1997 Feb;179(4):1413-6.
doi: 10.1128/jb.179.4.1413-1416.1997.

Role of His243 in the phosphatase activity of EnvZ in Escherichia coli

K Skarphol  1 , J Waukau, S A Forst
Affiliations

Role of His243 in the phosphatase activity of EnvZ in Escherichia coli

K Skarphol et al. J Bacteriol. 1997 Feb.

Abstract

EnvZ undergoes autophosphorylation at His243 and subsequently transfers the phosphate group to OmpR. EnvZ also possesses an OmpR-phosphate phosphatase activity. We examined the role of His243 in the phosphatase function by replacing His with either Val, Tyr, Ser, Asp, or Asn. EnvZH243V and EnvZH243Y were both shown to possess phosphatase activity in vitro. In addition, the mutant proteins were able to reduce the high level of OmpR-phosphate present in the envZ473 strain. These results indicate that His243 of EnvZ is not essential for stimulating the dephosphorylation of OmpR-phosphate.

PubMed Disclaimer

References

    1. Proc Natl Acad Sci U S A. 1988 Dec;85(23):8919-23 - PubMed
    1. J Biol Chem. 1996 Jan 19;271(3):1424-9 - PubMed
    1. Proc Natl Acad Sci U S A. 1989 Aug;86(16):6052-6 - PubMed
    1. Genes Dev. 1989 Nov;3(11):1725-34 - PubMed
    1. J Bacteriol. 1990 Jun;172(6):3473-7 - PubMed

Publication types

MeSH terms

LinkOut - more resources