Pluripotency and differentiation of embryonic stem cell lines from the medakafish (Oryzias latipes)

Abstract

Small aquarium fish, like the medaka and zebrafish, offer an excellent opportunity to combine embryological, genetic and molecular analyses of vertebrate development. Pluripotent embryonic stem (ES) cells have enormous potential to study the totipotency and differentiation of cells and provide s bridge linking in vitro manipulations of the genome. In this report we describe the establishment, pluripotency and differentiation of medaka ES-like cell lines (MES). The MES cells exhibit stable growth over 18 months of culture with 100 passages using defined culture conditions in the absence of feeder layer cells. They have a normal karyotype and form colonies of densely packed, alkaline phosphatase-positive cells resembling undifferentiated mouse ES cells. In suspension culture they form embryoid bodies, and under appropriate conditions, differentiate into a variety of cell types.