Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1997 Jan 1;244(1):80-5.
doi: 10.1006/abio.1996.9869.

Synthesis and luminescence spectral characterization of long-lifetime lipid metal-ligand probes

L Li  1 , H Szmacinski, J R Lakowicz
Affiliations

Synthesis and luminescence spectral characterization of long-lifetime lipid metal-ligand probes

L Li et al. Anal Biochem. .

Erratum in

  • Anal Biochem 1997 May 1;247(2):465

Abstract

We synthesized phospholipid analogues of phosphatidyl ethanolamine which contains a ruthenium metal-ligand complex (MLC) covalently bound to the amino group. Two analogues were synthesized, containing either one (Ru-PE) or two (Ru-PE2) lipid molecules covalently linked to the MLC by the amino group of the lipid. These MLC-lipid probes display intensity decay times from 682 to 357 ns, depending on temperature. Importantly, the luminescence MLC groups display polarized emission, enabling their use for studies of membrane dynamics. The long intensity decay times allowed measurement of the overall rotation correlation time of lipid vesicles to several microseconds. The spectral properties of the model membranes containing Ru-PE or Ru-PE2 were independent of the probe-to-lipid molar ratio from 1:20 to 1:100, suggesting minimal tendency for probe-probe interactions. These MLC-lipid probes can be expected to have numerous applications in studies of membrane dynamics on the microsecond timescale.

PubMed Disclaimer

Figures

FIG. 1.
FIG. 1.
Absorption and emission spectra of Ru-PE (—) and Ru-PE2 (- - -) in DPPG vesicles at 20°C. Also shown is the excitation anisotropy spectra of the parent compounds Ru(bpy)2(mcbpy)+1 (—) and Ru(bpy)2(dcbpy) (- - -) in glycerol/water at −55°C.
FIG. 2.
FIG. 2.
Frequency-domain intensity decays of MLC–PE and MLC–PE2 in DPPG vesicles at various temperatures.
FIG. 3.
FIG. 3.
Temperature-dependent steady state anisotropies of DPPG vesicles labeled with Ru–PE or Ru–PE2.
FIG. 4.
FIG. 4.
Frequency-domain anisotropy decays of Ru–PE in DPPG vesicles.
FIG. 5.
FIG. 5.
Frequency-domain anisotropy decays of Ru–PE2 in DPPG vesicles.
SCHEME I.
SCHEME I.
Structures of the two MLC–lipid probes: Ru(bpy)2(mcbpy)-PE (Ru–PE) and Ru(bpy)2(dcpby)-PE2(Ru–PE2).
See this image and copyright information in PMC

References

    1. Stubbs CD, and Williams BW (1992) in Topics in Fluores cence Spectroscopy, Vol. 3, Biochemical Applications (Lakowicz JR, Ed.), pp. 231–271, Plenum, New York.
    1. Dewey TG (Ed.) (1991) in Biophysical and Biochemical Aspects of Fluorescence Spectroscopy, p. 294, Plenum, New York.
    1. Davenport L, Knutson JR, and Brand L (1989) in Subcellu lar Biochemistry, (Harris JR, and Etemadi AH, Eds.), pp. 145–188, Plenum, New York.
    1. Shinitzky M, Dianoux AC, Gitler C, and Weber G (1971) Biochemistry 10, 2106–2113. - PubMed
    1. Cogen U, Shinitzky M, Weber G, and Nishida T (1973) Bio chemistry 12, 521–528. - PubMed

Publication types

MeSH terms