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. 1996 Dec;22(12):891-3.

[Expression of synthetic gene for human angiogenin in E. coli cells]

[Article in Russian]
  • PMID: 9054338

[Expression of synthetic gene for human angiogenin in E. coli cells]

[Article in Russian]
A A Nikonova et al. Bioorg Khim. 1996 Dec.

Abstract

A synthetic gene for human angiogenin was cloned into pRTang under control of the Proteus mirabilis recA promoter. After induction with mitomycin C, Escherichia coli cells transformed with pRTang produced an additional 14.2-kDa protein. According to electrophoretic and immunochemical analyses, this protein was identical to human angiogenin.

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