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. 1976 Jul 1;157(1):233-5.
doi: 10.1042/bj1570233.

Iron-dependent binding of 8-anilinonaphthalene-1-sulphonate by both lactoferrin and transferrin

N Bilić, M Casey, B Blanc

Iron-dependent binding of 8-anilinonaphthalene-1-sulphonate by both lactoferrin and transferrin

N Bilić et al. Biochem J. .

Abstract

Fluorescence of 8-anilinonaphthalene-1-sulphonate is enhanced by both lactoferrin and transferrin. The enhancement is relatively low between pH 4 and 8, and high at below pH 4. At physiological pH the fluorescence enhancement is higher with the iron-deprived than with the iron-saturated proteins. Binding of iron by lactoferrin is associated with lowering affinity for the dye.

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