The use of antibodies to study the architecture and developmental regulation of plant cell walls

Abstract

This review covers the generation and use of antibodies to defined components of plant and algal cell walls and how these have contributed to our understanding of the spatial and developmental regulation of cell walls. Particular emphasis is placed upon the generation and characterization of monoclonal antibodies to matrix polysaccharides, extensins, and arabinogalactan-proteins of higher plants, and algal polysaccharides and glycoproteins. Immunolocalization studies are discussed in relation to the identification of molecular domains within cell walls, cell adhesion, cell differentiation, and the establishment of plant interactions with other organisms.