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. 1997 Jan;14(1):13-7.
doi: 10.1023/a:1018500712733.

Chemical synthesis of Haemophilus influenzae glycopeptide conjugates

A A Kandil  1 , N Chan, M Klein, P Chong
Affiliations

Chemical synthesis of Haemophilus influenzae glycopeptide conjugates

A A Kandil et al. Glycoconj J. 1997 Jan.

Abstract

A simple procedure for conjugating synthetic fragments of the capsular polysaccharide of Haemophilus influenzae type b, poly-3-beta-D-ribose-(1,1)-D-ribitol-5-phosphate (sPRP) to linear peptides is described. The procedure consists of (i) reacting the amino group of amino-heptyl sPRP with m-maleimidobenzoyl-N-hydroxysuccinimide (MBS) in phosphate buffer, pH 7.5; (ii) selectively coupling the MBS-modified sPRP to the sulfhydryl group of the cysteine residue of peptides containing functional T-helper cell epitope(s). The glycopeptide conjugates were purified by gel filtration chromatography, biochemically characterized, and elicited protective level of anti-PRP antibody responses in rabbits.

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