The urinary excretion of the nucleosides pseudouridine and 1-methylinosine during normal menstrual cycle

Abstract

The excretion levels of the nucleosides pseudouridine and 1-methylinosine were determined by gas-liquid chromatography in 24-h urine specimens from young women during normal menstrual cycle. These nucleosides are derived primarily from transfer RNA and their excretion reflects the turnover of tRNA. The excretion levels were found to be essentially unaltered by the cycle and the average excretion values with standard deviations were 0.70 +/- 0.078 and 0.051 +/- 0.011 mg/kg/24 h for pseudouridine and 1-methylinosine, respectively.