The effects of voluntary exercise and immobilization on humoral immunity and endocrine responses in rats

Abstract

This research examined the effect of type of stressor (physical vs. psychological) on humoral immunity and neuroendocrine responses in male and female rats. Eighty adult Sprague-Dawley rats were assigned to one of the four stress conditions (n = 20 animals/group): 1. Voluntary running (high physical/low psychological stress); 2. immobilization (low physical/high psychological stress); 3. mixed stress (running and immobilization); and 4. cage control group. The experimental manipulations were conducted over a 6-week period for 4 h/day. Five weeks after the start of the study, all animals were immunized with 1 ml of a 10% suspension of sheep red blood cells (SRBC) in saline and sacrificed 1 week later. Data analyses revealed no main effect of stress on any of the immune or endocrine parameters. However, strong gender differences emerged within the stress conditions on these physiological parameters. The stressed female rats displayed an enhanced antibody response to SRBC and a higher percentage of peripheral blood lymphocytes than their male counterparts. However, there were no significant differences between the male and female control animals with respect to these variables. Female rats consistently displayed elevated levels of plasma corticosterone and adrenal norepinephrine across all conditions. In addition, female rats displayed heavier relative organ weights (adrenal and spleen). Taken together, the notion of differential immunity with respect to physical or psychological stress is not supported by the present study.