Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1997 Mar 15;25(6):1317-8.
doi: 10.1093/nar/25.6.1317.

B lymphocyte-specific, Cre-mediated mutagenesis in mice

R C Rickert  1 , J Roes, K Rajewsky
Affiliations

B lymphocyte-specific, Cre-mediated mutagenesis in mice

R C Rickert et al. Nucleic Acids Res. .

Abstract

Adaptation of the P1 phage-derived Cre /loxP site- specific recombination system to the gene targeting technique allows for the conditional deletion of genes in mice. To selectively modify genes in B lymphocytes, we have generated mice (designated CD19-Cre) which express cre under the transcriptional control of the B lineage-restricted CD19 gene. In a model system involving the cross of CD19-Cre mice with mice bearing a loxP -flanked substrate, we find a deletion efficiency of 75-80% in bone marrow-derived pre-B cells that increases to 90-95% in splenic B cells.

PubMed Disclaimer

References

    1. Nature. 1995 Jul 27;376(6538):352-5 - PubMed
    1. Science. 1995 Sep 8;269(5229):1427-9 - PubMed
    1. Nature. 1995 Dec 14;378(6558):720-4 - PubMed
    1. Proc Natl Acad Sci U S A. 1995 Dec 19;92(26):12070-4 - PubMed
    1. Nucleic Acids Res. 1995 Dec 25;23(24):5080-1 - PubMed

Publication types

MeSH terms