Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1977 Jul;4(7):2213-22.
doi: 10.1093/nar/4.7.2213.

DNA regions essential for the function of a bacteriophage fd promoter

T Okamoto, K Sugimoto, H Sugisaki, M Takanami
Free PMC article

DNA regions essential for the function of a bacteriophage fd promoter

T Okamoto et al. Nucleic Acids Res. 1977 Jul.
Free PMC article

Abstract

The promoter for the major coat protein gene of bacteriophage fd contains a unique sequence. TATAAT, in the non-transcribed region corresponding to the Pribnow box. A R-Hha I cleavage site which destroys functions is located five pairs upstream from the TATAAT sequence (fifteen base pairs upstream from the RNA initiation site). The promoter was cleaved into two fragments by R-Hha I and each promoter fragment was joined to DNA fragments derived from other regions. Ligation of the TATAAT-containing fragment to any of the DNA fragments examined resulted in recovery of promoter function. The results suggest for this type of promoter that no unique sequence is necessary upstream from the R-Hha I cleavage site although a contiguous DNA chain must be present in this area.

PubMed Disclaimer

References

    1. Nature. 1977 Jan 13;265(5590):117-21 - PubMed
    1. Proc Natl Acad Sci U S A. 1976 Nov;73(11):4174-8 - PubMed
    1. Nucleic Acids Res. 1975 Nov;2(11):2091-100 - PubMed
    1. J Mol Biol. 1974 Jan 5;85(4):475-84 - PubMed
    1. Nature. 1974 May 17;249(454):221-3 - PubMed