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. 1977 Aug;4(8):2561-72.
doi: 10.1093/nar/4.8.2561.

A simple, general procedure for purifying restriction endonucleases

T A Bickle, V Pirrotta, R Imber
Free PMC article

A simple, general procedure for purifying restriction endonucleases

T A Bickle et al. Nucleic Acids Res. 1977 Aug.
Free PMC article

Abstract

A simple, general method for purifying restriction endonucleases is described. The method employs precipitation of nucleic acids from crude extracts with polyethyleneimine followed by affinity chromatography on columns of heparin covalently linked to agarose. Most of the sixteen enzymes tested could be purified to a degree sufficient for DNA sequencing work by this method sometimes supplemented by at most one step of ion exchange chromatography.

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References

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