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. 1977 Sep;4(9):3097-108.
doi: 10.1093/nar/4.9.3097.

Methylation of nucleosomal and nuclease sensitive DNA

R L Adams, E L McKay, J T Douglas, R H Burdon
Free PMC article

Methylation of nucleosomal and nuclease sensitive DNA

R L Adams et al. Nucleic Acids Res. 1977 Sep.
Free PMC article

Abstract

The proportion of cytosines methylated in the DNA of nucleosome oligomers and of core particles appears indistinguishable from that of total nuclear DNA from CHO cells. However the DNA in nucleoprotein which is initially released from nuclei by treatment with very low levels of micrococcal nuclease and the first 10% of material rendered acid soluble by treatment of nuclei with DNase I are enriched 2 fold in their content of 5 methylcytosine. (Cessation of hydrolysis by nuclease occurs concomitantly with precipitation of nucleosomal core particles).

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References

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